Chemical-induced DNA Damage and Human Cancer Risk

4th Monday, 2013  |   Others  |  no comments

Poirier MC. Discovery Medicine. Oct. 2012 Vol.14, No.77, Pp.283-8. discovery_medicine_no_77_miriam_c_poirier_table_1 For more than 200 years human cancer induction has been known to be associated with a large variety of chemical exposures. Most exposures to chemical carcinogens occur as a result of occupation, pollution in the ambient environment, lifestyle choices, or pharmaceutical use. Scientific investigations have revealed that the majority of cancer causing chemicals, or chemical carcinogens, act through “genotoxic” or DNA damaging mechanisms, which involve covalent binding of the chemical to DNA (DNA adduct formation). Cancer-inducing exposures are typically frequent and/or chronic over years, and the accumulation of DNA damage or DNA adduct formation is considered to be a necessary requirement for tumor induction. Studies in animal models have indicated that the ability to reduce DNA damage will also result in reduction of tumor risk, leading to the hypothesis that individuals having the highest levels of DNA adducts may have an increased cancer risk, compared to individuals with the lowest levels of DNA adducts. Here we have reviewed twelve investigations showing 2- to 9-fold increased Relative Risks (RR) or Odds Ratios (OR) for cancer in (the 25% of) individuals having the highest DNA adduct levels, compared to (the 25% of) matched individuals with the lowest DNA adducts. These studies also provided preliminary evidence that multiple types of DNA adducts combined, or DNA adducts combined with other risk factors (such as infection or inflammation), may be associated with more than 10-fold higher cancer risks (RR = 34-60), compared to those found with

a single carcinogen. Taken together the data suggest that a reduction in human DNA adduct level is likely to produce a reduction in human cancer risk.

Transcriptional profiling of Chinese medicinal formula Si-Wu-Tang on breast cancer cells reveals phytoestrogenic activity.

4th Monday, 2013  |   Breast Cancer, Herb or Compound  |  no comments

The use of traditional herbal medicine is widespread in China and other Asian countries, and is also rapidly growing in Western countries [32]. Despite its long history of use, many questions remain to be answered, due to lack of mechanistic understanding. In this study, we demonstrated, using a whole human genome microarray approach, the phytoestrogenic mechanism for a popular formula Si-Wu-Tang (SWT). Three major points are highlighted: (1) this is the first study to use DNA microarray-based gene expression generic cialis analysis to reveal phytoestrogenic activity of herbal medicine. The similar approach can be used for other natural products; (2) The genomic data

were validated by the “gold standard” method of gene expression study – quantitative RT-PCR; and (3) the discovery was confirmed by pharmacological assays such as cell proliferation and estrogen receptor luciferase reporter assays on breast cancer cell lines. The results presented here are very important for many women taking SWT for various conditions and clinical practitioners who recommend the use of SWT or other CAM with similar phytoestrogenic activities. BACKGROUND: Si-Wu-Tang (SWT), comprising the combination of four herbs, Paeoniae, Angelicae, Chuanxiong and Rehmanniae, is one of the most popular traditional oriental medicines for women’s diseases. In our previous study, the microarray gene expression profiles of SWT on breast cancer cell line MCF-7 were found similar to the effect of beta-estradiol (E2) on MCF-7 cells in the Connectivity Map database. METHODS: Further data analysis was conducted to find the main similarities cialis generic and differences between the effects of SWT and E2 on MCF-7 gene expression. The cell proliferation assay on MCF-7 (ER-positive) and MDA-MB-231 (ER-negative) cells were used to examine such estrogenic activity. genericcialis-onlineed The estrogenic potency of SWT was further confirmed by estrogen-responsive element (ERE) luciferase reporter assay in MCF-7 cells. RESULTS: Many estrogen regulated genes strongly up-regulated by E2 were similarly up-regulated by SWT, e.g., GREB1, PGR and EGR3. Of interest with regard to safety of SWT, the oncogenes MYBL1 and RET were strongly induced by E2 but not by SWT. Quantitative RT-PCR analysis revealed a highly concordant expression change in cialis 10 mg selected genes with data obtained by microarrays. Further supporting SWT’s estrogenic activity, in MCF-7 but not in MDA-MB-231 cells, SWT stimulated cell growth at lower concentrations (CONCLUSIONS: These results revealed a new mechanism to support the clinical use of SWT for estrogen related diseases and possibly for cancer prevention. This study also demonstrated the feasibility of using microarray transcriptional profiling to discover phytoestrogenic components that are present in natural products. Source: Liu M, Fan J, Wang S, Wang Z, Wang C, Zuo Z, Chow MS, Shi L, Wen Z, Huang Y. BMC Complement Altern Med. 2013 Jan 10;13(1):11. References 1. Wang ZJ, Wo SK, Wang L, Lau CB, Lee VH, Chow MS, Zuo Z: Simultaneous quantification of active components in the herbs and products of Si-Wu-Tang by high performance liquid chromatography-mass spectrometry. J Pharm Biomed Anal 2009, 50(2):232–244. 2. Yeh LL, Liu JY, Lin KS, Liu YS, Chiou JM, Liang KY, Tsai TF, Wang LH, Chen CT, Huang CY: A randomised placebo-controlled trial of a traditional Chinese herbal formula in the treatment of primary dysmenorrhoea. PLoS One 2007, 2(1):e719. 3. Ohta H, Ni JW, Matsumoto K, Watanabe H, Shimizu M: Peony and its major constituent, paeoniflorin, improve radial maze performance impaired by scopolamine in rats. Pharmacol Biochem Behav 1993, 45(3):719–723.

Talk to other nursing moms, read books to familiarize

2nd Saturday, 2013  |   Uncategorized  |  no comments

The 24 suites are set in six (6) separate cottages, all with private balconies with an ottoman daybed. Nourishing, nurturing food unashamedly uncomplicated can be found at the resort restaurant, which uses seasonal, local and organic produce farm to table where possible. Guests will have time to re discover what nature has to offer since the dawn of time and reconnect with those simple luxuries that have been forgotten in day to day life.

replica hermes Some reach in coolers lack accessible, accurate thermometers. Wiping cloths not stored in sanitizing solution between uses. Interior of both ice machines not clean. These days in many public schools, math students are split up into two or more levels according to their ability. The intent is to be able to cater to the needs of students more precisely. For those students tracked in lower level classes, however, outcome was that students never got to have the opportunities the other kids did Replica Hermes, says Eric. replica hermes

hermes replica birkin Gossiped with Brodie. Watched movies and ate Wendy Cleaned my dining room and kitchen, including doing dishes, while actually enjying the fact that people were watching football in my living room. Cooked a Sunday meal. How should I prepare for breastfeeding?Learn as much as you can about breastfeeding even before your baby’s born. Talk to other nursing moms Replica Hermes Wallet, read books to familiarize yourself, call your local La Leche League International chapter Replica Hermes Bag, and consider taking a breastfeeding class (offered by most hospitals) some time in your last trimester. The more you know about how to get started and the benefits of nursing, the more likely you are to succeed at it.. hermes replica birkin

replica hermes birkin Am blessed to have lots and lots of family Replica Hermes Bag, Jane said. Are a lot of people that are alone and poor. You certainly have to feel something for the homeless. Last week, WRAP launched a pilot project in Bristol and Edinburgh to try to educate people to reuse their plastic bags more often. A similar campaign in Australia led to a 25 per cent reduction in use, so Swannell has great hopes for Britain. And we certainly have a long way to go.. replica hermes birkin

hermes replica bags Windows are usually made of clear plastic or colored plastic depending on the preferences of the bride and groom. It may be a good idea to have the candies as multicolored if you have a solid colored box. The clear window will show the pretty contrast of multicolored candies and solid box.. hermes replica bags

hermes bags replica Really are back, especially rose gold, Valley said. Been a hot new trend in the last couple of years, and I don see it dying out anytime soon. Knots and fabrics of every shape and size have dotted runways as spring collections debut.. If the Transportation Secretary, who oversees NHTSA, determines that the recalls are moving too slowly, the government can take over a recall. It can manage production, distribution and installation of replacement parts, including lining up other manufacturers to make more inflators. The government would send inflators to high humidity areas along the Gulf Coast first because the risk is highest there hermes bags replica.

In this 2 hour workshop, you will learn: WHAT IS YOGA?

25th Friday, 2013  |   Uncategorized  |  no comments

The story goes as follows: Seth (brother of Osiris) was jealous of Osiris and fought him to the death. After he killed Osiris he cut his body up into 14 pieces and spread the pieces throughout Egypt. Isis (Osiris’ wife) found out that her husband was killed and she searched egypt looking for his body parts.

Replica Hermes Birkin Adam Singleton, senior vice president at Financial Risk Management (FRM), emphasises that returns from any particular hedge fund strategy are cyclical. He says: “The relative strength of different hedge fund strategies changes over time. By holding exposure to many different hedge fund strategies and also shifting exposure to concentrate on higher performing strategies Replica Hermes Belt}, an investor can attempt to increase returns and avoid underperformance.”. Replica Hermes Birkin

Replica Hermes Birkin Bags Fast forward many decades and technology once again is upending the way New Orleans’ oldest restaurant does business. Alciatore might be horrified to learn that Antoine’s has now embraced online reservation service OpenTable. And that’s just one of the changes as the restaurant celebrates its 175th anniversary and strives to balance the old with the new.. Replica Hermes Birkin Bags

(Photo: Dorothy Edwards/Naples Daily News)LaBelle, Fla. Brian Wood runs a slaughterhouse here Replica Hermes Belt} {Hermes Belt Replica {Hermes Belt Replica, but not the typical kind. His business is alligators. “We all are going to get older. It’s not about me looking forward to it, it’s about me being OK with it Replica Hermes Belt,” he says. “I’ve learned how to live my life more fully from the women I have met who are 100 and still doing Pilates.

Replica Hermes Bags Curious about YOGA? Never taken a yoga class? Not sure what to expect? This is the workshop for you! You learn the basics of yoga, get your questions answered and experience a slow paced beginning level yoga class. All levels of fitness are welcome and no experience or special equipment required. In this 2 hour workshop, you will learn: WHAT IS YOGA? Introduction to yoga foundation, history and philosophy and benefits of Yoga PRANAYAMA TECHNIQUES Using the breath to relax the body and calm the mind ALIGNMENT PRINCIPALS How to practice yoga safely FOUNDATIONAL POSTURES An introduction to basic yoga postures INTRODUCTION TO PROPS Getting to know the tools that will help your practice. Replica Hermes Bags

We find this cosmic intent corroborated by Witzel. His reading of the references to oceans and seas is closer to the mark than Frawley’s. He quotes Rigvedic references to the four oceans’ Replica Hermes Belt}, or the eastern and western oceans’; or else the Atharvaveda northern {Hermes Belt Replica, upper ocean’.

Replica Hermes Stair tickets cannot be purchased online in advance, but most visitors find it too daunting a prospect, meaning the queues are much shorter. See our full expert Eiffel Tower ticket and queuing guide.3. Joining the Mona Lisa scrum. HMS Illustrious is a light aircraft carrier of the Invincible class operating for the British Royal Navy. Its pennant number is R06. It was built by the Swan Hunter shipbuilding company in the UK and launched on December 14, 1978 Replica Hermes.

And we know Katerecently looked amazing in this

25th Friday, 2013  |   Uncategorized  |  no comments

We also know thatPrince Williamapparently now isthe family chauffeur. And we know Katerecently looked amazing in this. Oh, and in this. The lens color does not impact the UV protection ability of sunglasses. Green and gray shades are good for general purpose use, whereas blue, brown, and amber may alter the perception of color a little. The darkness of a lens should be such that in bright light, your eyes are just about visible..

cheap oakley sunglasses Is an immigration hard liner who likes Trump idea to build a wall and Marino, who represents a conservative, more rural section of northeastern Pennsylvania fake oakley sunglasses, told the online publication Politico endorsing Trump was of my life changing moments. Post story said early endorsers are playing a pivotal role in helping the nominee expand his appeal in Washington. They even hold weekly strategy meetings with Trump campaign (that have become standing room only) to talk policy and politics. cheap oakley sunglasses

fake oakley sunglasses It’s a truly special retreat, and its location in Rutherford is convenient for exploring Napa Valley.8 of 10Travaasa Austin is set on its own nature preserve on a hilltop amid forested canyons, 20 miles west of downtown Austin. Its 72 rooms are housed in seven buildings built on three different levels, with correspondingly different views from their balconies. Hiking trails, an infinity edge pool enclosed by a wooden deck, a luxurious spa, and a restaurant with panoramic views are just some of the amenities providing inspiration and relaxation for visitors. fake oakley sunglasses

cheap oakleys Using a small prying tool, the pair flipped the lock on the door and entered with guns drawn. They ordered the women into the vault and secured their wrists with plastic ties, then quickly began stuffing money into duffle bags. Within minutes, using a metal cart to help transport the unwieldy stack of bags, they pushed through the door and loaded their stolen Jeep Cherokee with $4,461,681 in bundled cash. cheap oakleys

replica oakley sunglasses Some states, including New York, mandate that customers give companies a copy of their prescription to verify its timeliness and specifics. But in many cases, consumers can type in the prescriptions themselves, opening huge potential for errors. Dr. replica oakley sunglasses

replica oakleys Where is Louisa Boren Park again? I fumble around on the phone map only to conclude that it’s over there ish, past the museum and a playground and across Lakeview Cemetery. The graveyard is the resting place of father and son actor martial artists Bruce and Brandon Lee, as well as many of Seattle’s prominent early families. The names on many of the headstones mirror those on some of the city’s streets: Terry, Bell, Denny, Boren replica oakleys.