Herbs That Influence COX-2 Expression

Sunday, 26/12/2010  |   Herb or Compound  |  no comments

Chuan Xiong-Ligusticum Chuanxiong
Essential Oil of Ligusticum Chuanxiong Hort’s Influence on Expressions of COX-2 in Hypothalamus of Fever Rat
Objective: to investigate the antipyretic mechanism of Essential oil of Ligusticum chuanxiong Holt (CH). Methods: The expressions of COX -2 protein in hypothalamus of brewer’s yeast-induced fever rats were checked using Immunohistochemistry. Results: The expressions of COX -2 protein in hypotbalamus of model team are higher than normal team, expressions of teams of high and middle dose of CH are lower than model team, expression of team of low dose of CH has no obvious different with model team. Conclusion: one of the anfipyretie mechanism of essential oil of Ligustieum ehuanxiong Hort. (CH) may be to inhibit the expression of COX-2 protein in hypothalamus of rats, thereby reduce the content of PGE2 that lower the set point and produce anfipyrefie effect (Yang et al 2008).

Cui Yun Cao-Selaginella uncinata
Inhibiting Action of Total Flavones from Selaginella uncinata on COX-2 mRNA Expression in HT-29 Cells
Objective: To investigate the action of total flavones from Selaginella uncinata ( Desv. ) Spring on cyclooxygenase-2 (COX-2) mRNA expression in carcinoma of colon cell line HT-29 ,and explore related molecular mechanism thereby. Method: Total flavones from Selaginella uncinata was used on TH-29 cells and there were four groups including high-dose group, midst-dose group, low-dose group and negative control group. The morphological change of the cells was observed by inverted microscope. The expressions of COX-2 mRNA were measured using reverse transcription polymerase chain reaction (RT-PCR) method. Caraphoresis gel photo auto- analysis system was used to detect the products. Result: Comparing with the negative control group, the total flavonoids groups could significantly inhibit the expressions of the COX-2 mRNA level in a dose-dependent manner. Conclusion: It can be concluded that the mechanisms may be that the total flavonoids can inhibit COX-2 in mRNA level. Then it can inhibit COX-2 protein expression. It may be one of the anti-tumor mechanisms of Selaginella uncinata (Sun, Chen & Liu, 2010).

Deng Long Guo-Physalis peruviana
Supercritical carbon dioxide extract exhibits enhanced antioxidant and anti-inflammatory activities of Physalis peruviana.
Physalis peruviana L. (PP) is a medicinal herb widely used in folk medicine. In this study, supercritical carbon dioxide (SFE-CO2) method was employed to obtain three different PP extracts, namely SCEPP-0, SCEPP-4 and SCEPP-5. The total flavonoid and phenol concentrations, as well as antioxidant and anti-inflammatory activities of these extracts were analyzed and compared with aqueous and ethanolic PP extracts. Among all the extracts tested, SCEPP-5 demonstrated the highest total flavonoid (234.63+/-9.61 mg/g) and phenol (90.80+/-2.21 mg/g) contents. At concentrations 0.1-30 microg/ml, SCEPP-5 also demonstrated the strongest superoxide anion scavenging activity and xanthine oxidase inhibitory effect. At 30 microg/ml, SCEPP-5 significantly prevented lipopolysaccharide (LPS; 1 microg/ml)-induced cell cytotoxicity in murine macrophage (Raw 264.7) cells. At 10-50 microg/ml, it also significantly inhibited LPS-induced NO release and PGE2 formation in a dose-dependent pattern. SCEPP-5 at 30 microg/ml remarkably blocked the LPS induction of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression. Taken together, these results suggest that SCEPP-5, an extract of SFE-CO2, displayed the strongest antioxidant and anti-inflammatory activities as compared to other extracts. Its protection against LPS-induced inflammation could be through the inhibition of iNOS and COX-2 expression (Wu et al 2006).

References:
Meyskens CA et al. Chemoprevention of nonmelanoma skin cancer with celecoxib: A randomized, double-blind, placebo-controlled trial. J Natl Cancer Inst 2010 Nov 29; [e-pub ahead of print]. (http://dx.doi.org/10.1093/jnci/djq442)

Meyskens FL Jr and McLaren CE. Chemoprevention, risk reduction, therapeutic prevention, or preventive therapy? J Natl Cancer Inst 2010 Nov 29; [e-pub ahead of print]. (http://dx.doi.org/10.1093/jnci/djq466)

Sun Yingzhen; Chen Keli & Liu Zhen. Zhong Guo Yao Shi. 2010; (2): 163-164.

Wu SJ, Tsai JY, Chang SP, Lin DL, Wang SS, Huang SN, Ng LT. J Ethnopharmacol. 2006 Dec 6;108(3):407-13. Epub 2006 Jun 2

Yang Jin-rong, SONG Jun, HU Rong, Li Zulun, Zhang Quansen. Cheng Du Zhong Yi Yao Da Xue Xue Bao. 2008; 31(3): 38-39.

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